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KMID : 0950020050310020091
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Journal of Health Science & Medical Technology
2005 Volume.31 No. 2 p.91 ~ p.98
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Regulation of Interleukin-1β-induced Dedifferentiation and Apoptosis via p38 Mitogen-activated Protein Kinase pathway in Articular Chondrocytes
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Hur Jeong-Eun
Lee Chan-Jin
Kim Soon-Ki
Kim Na-Rae
Kim Hyun-Ju
Jo Kyung-Jin
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Abstract
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Interleukin-1 (IL-1)β in articular chondrocytes regulates differentiation, apoptosis, and inflammatory responses. It is still controversial, however, whether IL-1β induces chondrocytes dedifferentiation and death. We investigated the role of the mitogen-activated protein kinase (MAPK) subtypes on IL-1β-induced dedifferentiation and apoptosis, as indicated by the inhibition of type II collagen expression and proteoglycan synthesis of rabbit articular chondrocytes. IL-1β treatment did not affect activation of ERK-1/2, but stimulation of p38 kinase. Inhibition of phospho ERK-1/2 with PD98059 enhanced IL-1β-induced dedifferentiation, and apoptosis up to 13.5%, whereas inhibition of phospho p38 kinase with SB203580 inhibited dedifferentiation, and apoptosis. Our results indicate that SB203580, p38 kinase inhibitor, inhibits IL-1β-induced dedifferentiation, and apoptosis.
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KEYWORD
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Interleukin-1(IL-1)β, chondrocytes, dedifferentiation, mitogenactivated protein kinase(MAPK), apoptosis
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